Olive oil cultivation and production has been a traditional agricultural activity maintained for centuries in Greece and other Mediterranean countries such as Italy, Spain, Portugal, etc. The production of olive oil is located mostly at the southern part of Greece and especially Crete and Peloponnese. Olive oil is a valuable good with high nutritional and biological value. However, the process of cultivation and production of olive oil may have adverse effects on the environment in several stages during the life cycle, if not managed properly. This is partly linked to the use of fertilizers and pesticides during cultivation, irrigation matters, waste generation, and the processes taking place during olive oil production.